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easysep mouse ilc2 enrichment kit (STEMCELL Technologies Inc)

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STEMCELL Technologies Inc easysep mouse ilc2 enrichment kit
Easysep Mouse Ilc2 Enrichment Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/easysep+mouse+ilc2+enrichment+kit/easysep+mouse+ilc2+enrichment+kit/pm40628263-425-14-19
Average 90 stars, based on 1 article reviews

easysep mouse ilc2 enrichment kit - by Bioz Stars, 2026-08

90/100 stars

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ILC2s formed cell clusters with CD8 + T cells in the presence of antigen. (a) the cells showing red fluorescence are ILC2s, whereas those with no fluorescence are CD8 + T cells. ILC2s (2 × 10 were cocultured with CD8 + T (6 × 10 cells for 20 hours. Live cell imaging of ILC2–CD8 + T-cell interactions at different time points revealed that the cells formed constant cell clusters during the coculture. Scale bar = 50 μm or 5 μm. (b) Statistical analysis of cell cluster number per field ( n = 4 views per well). (c) Statistical analysis of cell cluster area percentage per field ( n = 4 views per well). Data are shown as the means ± SEMs. The p value was determined by two-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant.

Journal: Oncoimmunology

Article Title: Group 2 innate lymphoid cells boost CD8 + T-cell activation in anti-tumor immune responses

doi: 10.1080/2162402X.2023.2243112

Figure Lengend Snippet: ILC2s formed cell clusters with CD8 + T cells in the presence of antigen. (a) the cells showing red fluorescence are ILC2s, whereas those with no fluorescence are CD8 + T cells. ILC2s (2 × 10 were cocultured with CD8 + T (6 × 10 cells for 20 hours. Live cell imaging of ILC2–CD8 + T-cell interactions at different time points revealed that the cells formed constant cell clusters during the coculture. Scale bar = 50 μm or 5 μm. (b) Statistical analysis of cell cluster number per field ( n = 4 views per well). (c) Statistical analysis of cell cluster area percentage per field ( n = 4 views per well). Data are shown as the means ± SEMs. The p value was determined by two-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant.

Article Snippet: To obtain murine ILC2s, mouse lungs were prepared as described in Tissue Processing, and the suspension was enriched for ILC2s using EasySep TM Mouse ILC2 Enrichment Kit (STEMCELL Technologies) according to the manufacturer’s protocols.

Techniques: Fluorescence, Live Cell Imaging

OVA-loaded ILC2s increased CD8 + T cells cytotoxicity and delayed antigen-specific tumor growth. (a) Schematic diagram of the experimental design. Activated ILC2s were loaded with OVA protein or MOG protein. The ratio of OT-I CD8 + T cells to tumor cells was 10:1. (b) Representative data for LDH release from B16F10-OVA cells after treatment with OT-I CD8 + T cells induced by ILC2s ( n = 3 independent wells). (c) Representative images of B16F10-OVA cells stained with crystal violet after co-culture with OT-I CD8 + T cells. Statistical analysis of the areas of living cells at the bottom of the plate was performed ( n = 3 views per well). Data are shown as the mean ± SEMs. The p value was determined using one-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. (d) B16F10-OVA(2 × 10 mixed with OVA loaded or not ILC2s (2 × 10 were inoculated subcutaneously until the maximum tumor reached ~1000 mm 3 . (e–f) Tumor growth curve and volume in different treatment groups ( n = 4–6 mice per group). Data are shown as the mean ± SEMs. The p value was determined using a two-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. (g–i) the percentages of CD8 + T cells and Treg cells and cell ratio in the tumors of ILC2- and OVA-loaded ILC2-treated mice and control mice ( n = 4–6 mice per group). Data are shown as the mean ± SEMs. The p value was determined by one-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant.

Journal: Oncoimmunology

Article Title: Group 2 innate lymphoid cells boost CD8 + T-cell activation in anti-tumor immune responses

doi: 10.1080/2162402X.2023.2243112

Figure Lengend Snippet: OVA-loaded ILC2s increased CD8 + T cells cytotoxicity and delayed antigen-specific tumor growth. (a) Schematic diagram of the experimental design. Activated ILC2s were loaded with OVA protein or MOG protein. The ratio of OT-I CD8 + T cells to tumor cells was 10:1. (b) Representative data for LDH release from B16F10-OVA cells after treatment with OT-I CD8 + T cells induced by ILC2s ( n = 3 independent wells). (c) Representative images of B16F10-OVA cells stained with crystal violet after co-culture with OT-I CD8 + T cells. Statistical analysis of the areas of living cells at the bottom of the plate was performed ( n = 3 views per well). Data are shown as the mean ± SEMs. The p value was determined using one-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. (d) B16F10-OVA(2 × 10 mixed with OVA loaded or not ILC2s (2 × 10 were inoculated subcutaneously until the maximum tumor reached ~1000 mm 3 . (e–f) Tumor growth curve and volume in different treatment groups ( n = 4–6 mice per group). Data are shown as the mean ± SEMs. The p value was determined using a two-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant. (g–i) the percentages of CD8 + T cells and Treg cells and cell ratio in the tumors of ILC2- and OVA-loaded ILC2-treated mice and control mice ( n = 4–6 mice per group). Data are shown as the mean ± SEMs. The p value was determined by one-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant.

Techniques: Staining, Co-Culture Assay, Control

Adoptive transfer of OVA-loaded ILC2s inhibited B16F10-OVA lung metastasis. (a) Schematic diagram showing strategy for OVA-loaded ILC2s adoptive transfer treatment in lung metastasis model. (b) Representative pictures of lung tumor clones. (c) Representative photomicrographs of haematoxylin/eosin(HE)-stained lung sections. (d) the metastatic tumor nodules in lung in different treatment group ( n = 6 mice per group). (e) the lung weight in different treatment group ( n = 3 mice per group). (f–h) the percentages of CD8 + T cells and Treg cells and cell ratio in the tumors of ILC2- and OVA-loaded ILC2-treated mice and control mice ( n = 3 mice per group). Data are shown as the means ± SEMs. The p value was determined by one-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant.

Journal: Oncoimmunology

Article Title: Group 2 innate lymphoid cells boost CD8 + T-cell activation in anti-tumor immune responses

doi: 10.1080/2162402X.2023.2243112

Figure Lengend Snippet: Adoptive transfer of OVA-loaded ILC2s inhibited B16F10-OVA lung metastasis. (a) Schematic diagram showing strategy for OVA-loaded ILC2s adoptive transfer treatment in lung metastasis model. (b) Representative pictures of lung tumor clones. (c) Representative photomicrographs of haematoxylin/eosin(HE)-stained lung sections. (d) the metastatic tumor nodules in lung in different treatment group ( n = 6 mice per group). (e) the lung weight in different treatment group ( n = 3 mice per group). (f–h) the percentages of CD8 + T cells and Treg cells and cell ratio in the tumors of ILC2- and OVA-loaded ILC2-treated mice and control mice ( n = 3 mice per group). Data are shown as the means ± SEMs. The p value was determined by one-way ANOVA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, not significant.

Techniques: Adoptive Transfer Assay, Clone Assay, Staining, Control

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